Immunomodulatory Effects of Calcium and Strontium Co-Doped Titanium Oxides on Osteogenesis
نویسندگان
چکیده
The effects of calcium (Ca) or strontium (Sr) on host osteogenesis and immune responses have been investigated separately. In clinical practice, these two elements may both be present around an orthopedic device, but their potential synergistic effects on osteogenesis and the immune response have not been explored to date. In this work, we investigated the immunomodulatory effects of Ca and Sr co-doped titanium oxides on osteogenesis in vitro using the mouse macrophage cell line RAW 264.7 alone and in co-culture with mouse bone mesenchymal stem cells (BMSCs), and in vivo using a mouse air-pouch model. Coatings containing Ca and Sr at different concentration ratios were fabricated on titanium substrates using micro-arc oxidation and electrochemical treatment. The in vitro and in vivo results demonstrated that the Ca and Sr concentration ratio has a marked influence on macrophage polarization. The coating with a Ca/Sr ratio of 2:1 was superior to those with other Ca and/or Sr concentrations in terms of modulating M2 polarization, which enhanced osteogenic differentiation of mouse BMSCs in co-culture. These findings suggest that the osteoimmunomodulatory effect of a titanium-oxide coating can be enhanced by modulating the concentration ratio of its components.
منابع مشابه
Morphology, Composition, and Bioactivity of Strontium-Doped Brushite Coatings Deposited on Titanium Implants via Electrochemical Deposition
Surface modification techniques have been applied to generate titanium implant surfaces that promote osseointegration for use in dental applications. In this study, strontium-doped brushite coatings were deposited on titanium by electrochemical deposition. The phase composition of the coating was investigated by energy dispersive X-ray spectroscopy and X-ray diffraction. The surface morphologie...
متن کاملUnusual Electronic Transport and Magnetism in Titanium Oxide Based Semiconductors and Metals
Title of dissertation: UNUSUAL ELECTRONIC TRANSPORT AND MAGNETISM IN TITANIUM OXIDE BASED SEMICONDUCTORS AND METALS Shixiong Zhang, Doctor of Philosophy, 2007 Dissertation directed by: Professor T. Venky Venkatesan Department of Physics The main objective of this thesis was to explore the structural, electrical, magnetic and optical properties of titanium based novel oxide thin films, such as t...
متن کاملMethane Combustion and CO Oxidation on Ag-Doped LaMn0.8Cu0.2O3±δ Mixed Oxides Prepared by Pechini and Sol–Gel Methods
متن کامل
Multifunction Sr, Co and F co-doped microporous coating on titanium of antibacterial, angiogenic and osteogenic activities
Advanced multifunction titanium (Ti) based bone implant with antibacterial, angiogenic and osteogenic activities is stringently needed in clinic, which may be accomplished via incorporation of proper inorganic bioactive elements. In this work, microporous TiO2/calcium-phosphate coating on Ti doped with strontium, cobalt and fluorine (SCF-TiCP) was developed, which had a hierarchical micro/nano-...
متن کاملAuto - combustion synthesis of perovskite - type oxides
A versatile one-pot auto-combustion method for the synthesis of powders of iron-doped strontium titanate, SrTi1-xFexO3-δ, has been developed. The synthesis is optimised by the combined use of EDTA and citric acid as chelating agents, and an appropriate balance between fuel and oxidising elements in the reaction mixture. The method produces immediately an almost phase-pure perovskite oxide powde...
متن کامل